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The use of gross pathology of sh is well established as an integral part of the suite of biomarkers used in biological effects of contaminants monitoring programmes in Europe. Gross pathology recorded according to International Council for the Exploration of the Sea (ICES) guidelines includes acute and healing ulcers, lymphocystis, epidermal hyperplasia/ papilloma and the presence of liver nodules (ICES, 1996). Ulcerations are likely to result from a variety of causes, including physical trauma and subsequent infection with bacteria and other opportunistic organisms. Lymphocystis disease is known to be caused by an iridovirus. Infected cells become greatly hypertrophied, forming clusters of nodules on the surface of the sh (Bucke et al., 1983). Occasionally, lymphocystis nodules occur on the gills and in internal organs. At present, there does not appear to be a strong link between xenobiotic exposure and lymphocystis disease in at sh (M ller, 1990), but there are seasonal and annual variations (Riersen & Fugelli, 1984). Epidermal hyperplasia and papilloma have been recorded from many sh species from contaminated and relatively clean environments (Cross, 1986; Baumann et al., 1987; Hayes et al., 1990; Bowser et al.,

We have discussed how files can be used for the implementation of storage homes and storage object incarnations. The usage of files for achieving persistence, however, is overly simplistic. File systems usually do not support concurrency control and transactional access.

for one spouse s moving out Sometimes, an arrangement can be made where the spouses take turns living in the house or other temporary housing If the parties agree not to bring in lawyers right away, the process is far more likely to remain nonadversarial, and the therapist can better help the couple to maintain or achieve a nonblame attitude that is far more conducive to personal and familial healing The therapist basically continues to do couple counseling The couple should be informed that more than half of couples who decide to physically separate do go on to divorce Knowing this, the noninitiator may strongly oppose a separation, fearing it signals the reality of a divorce Mixed messages about separating may be given by both parties, and the therapist needs to help the couple not only sort out those messages, but to explore the advantages and disadvantages of a separation.

until a single cluster containing the whole set is obtained In each iteration, the two clusters that are chosen for joining are two clusters with the closest distance to each other The result of hierarchical clustering is a binary tree; descendants of each cluster in that tree are the two subclusters of which the cluster consists The distance between two clusters in the tree re ects their correlation distance Hierarchical clustering provides a visualization of the relationships between gene expression pro les (see Fig 414) K-means clustering groups genes into a prespeci ed number of clusters by minimizing the distances within each cluster and maximizing the distances between clusters The K-means clustering method rst chooses k genes called centroids (which can be done randomly or by making sure that their expression pro les are very different).

It then examines all gene expression pro les and assigns each of these to the cluster with the closest centroid The position of a centroid is recalculated each time a gene expression pro le is added to the cluster by averaging all pro les within the cluster This procedure is iteratively repeated until stable clusters are obtained, and no gene expression pro les switch clusters between iterations The K-means method is computationally less demanding than hierarchical clustering However, an obvious disadvantage is the need for the selection of parameter k, which is generally not a trivial task 4352 Alternative Clustering Methods for Gene Expression Pro les Alternative clustering methods that are used with gene expression data include the self-organizing map (SOM) and random forest (RF) clustering An SOM is a clustering method implemented with a neural network and a special training procedure.

A legal separation involves essentially all the financial and custodial decisions of a divorce, but may be preferred by couples who feel there need to be very clear guidelines in these areas and who want the relief and security of having these decisions in place Whether a separation is a dress rehearsal or acknowledged prelude to divorce or an alternative attempt to change perceptions and save a marriage, it is important for the therapist and clients to go over the ground rules of the separation In this author s experience, amazingly few couples openly talk about all the practical ramifications and decisions of a separation, other perhaps than to discuss what happens with the children.